News in Biochemistry
نویسندگان
چکیده
A fiber optic biosensor based on DNA aptamers used as receptors was developed for the measurement of throm bin concentration. Anti thrombin DNA aptamers were immobilized on silica microspheres, placed inside microwells on the distal tip on an imaging optical fiber, coupled to a modified epifluorescence microscope through its proximal tip. Thrombin concentration is determined by a competitive binding assay using a fluorescein labeled competitor. The biosensor is selective and can be reused without any sensitivity change. The thrombin limit of detection is 1 nM, sample volume is 10 μl, and assay time per sample is 15 min including the regeneration step.
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